br Q Experiment similar to that shown in panel
(Q) Experiment similar to that shown in panel P but done with 100 mM Trolox. Values are the mean of four different NSCLC cell lines and normalized to untreated controls.
(legend on next page)
Figure S7. Glycolysis Mediates Antioxidant- and BACH1-Induced Migration and Metastasis, Related to Figures 6 and 7
(A) Top, western blots showing amounts of HK2 and the loading control ACTIN in A549 KX2-391 transduced with a control plasmid or a plasmid encoding human HK2. Bottom, glycolysis rates.
(B) Transwell migration of A549 cells from panel A.
(C) Glycolysis-derived (left) and total (right) ATP production rates in A549 cells after incubation for > 7 days with 1 mM NAC.
(D) Steady-state total ATP levels in A549 cells after incubation for > 7 days with 1 mM NAC.
(E) Scratch-wound migration of mTC-SAM-sgTom and mTC-SAM-sgBach1 incubated with the glycolysis inhibitors 2-DG (1 mM) and lonidamine (LND, 50 mM).
(F) Scratch-wound migration of mTC and mTN cells incubated with 1 mM of the mitochondrial pyruvate transporter inhibitor UK5099.
(G) Lactate concentration in the medium of mTC and mTN cells incubated for 24 hr with the MCT-1 inhibitor AZD3965. Values are the mean of three cell lines per condition and normalized to untreated mTC.
(H) Viability of mTC and mTN cells incubated for 24 hr with AZD3965. Values are the mean of 3 cell lines per condition and normalized to untreated mTC.
(J) Kaplan-Meier graph of TCGA data showing survival of lung cancer (LUAD) patients with high and low GAPDH expression (Z-score = 2.0)
(K) Kaplan-Meier graph of TCGA data showing survival of lung cancer (LUAD) patients with high and low SLC16A1 expression (Z-score = 2.0)
(L) Scratch-wound migration of mTC and mTN cells incubated with increasing concentrations of the pentose phosphate pathway inhibitor 6-aminonicotinamide (6-AN).
Contents lists available at ScienceDirect
Chemical Engineering Journal
journal homepage: www.elsevier.com/locate/cej
Bacterial cellulose based composites enhanced transdermal drug targeting T for breast cancer treatment
Ling-Kun Zhanga,b, Shiwei Dua,b, Xiaozhen Wanga,b, Yuxuan Jiaoa,b, Liang Yina,b, Yi Zhanga,b, Yan-Qing Guana,b,
a School of Life Science, South China Normal University, Guangzhou 510631, China
b Institute for Advanced Materials and Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum Materials South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China
• The lasersensitive magnetic nano-particles were loaded into BC mem-brane.
• The drugs delivered transdermally under a magnetic field and laser irra-diation.
• This research provides solutions to enhance drug targeting transdermal delivery.
Nanotherapies combined with stimuli-triggered activated agents have become attractive ways to traditional chemotherapy. The Fe3O4 nanoparticles was employed as the core coated firstly with hydrogels of dually loaded both doxorubicin and hematoporphyrin monomethyl ether. Then, the folic acid was grafted onto the surface of the composites to construct a laser-sensitized magnetic nanoparticles (LMNs), followed by these LMNs being loaded into BC membrane. On the 14th day after treatment, tumor growth inhibition (TGI) was evaluated to 80.38% under the target system. When delivered transdermally under a magnetic field and laser, the synthesized BC/LMNs could oﬀer solutions to challenge in breast cancer treatment, including the limited penetration depth, which could facilitate the bypassing of the stratum corneum barrier and enhance transdermal drug delivery.
Breast cancer is a common life-threatening illness and a leading cause of death. The main treatments of the breast cancer currently in-clude surgery, chemotherapy, and radiotherapy [1–3]. But, these treatments have limited eﬃcacy, side eﬀects and toxicity. Therefore, alternative and eﬀective therapeutic approaches for the treatment of
In the research process of anticancer drug delivery for surface cancer treatment, the skin has emerged as an important alternative route, which would eliminate the need for conventional invasive ad-ministration procedures. In addition, localizing treatment could mini-mize undesirable systemic eﬀects and could prevent cases of pessimal stimulation and toxicity. However, the stratum corneum of the skin
Corresponding author at: School of Life Science, South China Normal University, Guangzhou 510631, China. E-mail address: [email protected] (Y.-Q. Guan).