br Reference br Bones of

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Bones of skull and face and Reference
Reference
associated joints
Histology
Conventional Chordoma Reference
Reference
Localized Reference
Reference
Yes Reference
Reference
Yes Reference
Reference
Reference
Yes Reference
Reference
There were six variables involving patient age, primary site, histology, tumor stage, use of surgery and tumor size which were related to OS (P<0.05), and WY14643 other variables lost significance. And six variables involving patient age, histology, tumor stage, tumor size, use of surgery and chemotherapy were related to CSS (P<0.05), and the other variables lost significance.
ACCEPTED MANUSCRIPT
CI, confidence interval; HR, hazard ratio.
ACCEPTED MANUSCRIPT
Table 3: Multivariate cox regression analysis of cancer-specific survival and Overall survival in the training cohort
Covariates Cancer-specific survival
Overall survival
Male Reference
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<38 Reference
Reference
Bones of skull and face and Reference
Reference
associated joints
Histology
Conventional Chordoma Reference
Reference
Localized Reference
Reference
Yes Reference
Reference
Yes Reference
Reference
Reference
Yes Reference
Reference
Age, primary site, histology, surgical stage, use of surgery and tumor size were identified as independent prognostic factors for both OS and CSS(P<0.05), and the other variables lost significance.
ACCEPTED MANUSCRIPT
CI, confidence interval; HR, hazard ratio.
ACCEPTED MANUSCRIPT
Table 4: Detailed scores of prognostic factors in the OS and CSS nomograms
Characteristic OS nomogram CSS nomogram
Age
Primary site
Bones of skull and face and associated joints 3.9 4.3
Pelvic bones, sacrum, coccyx and associated joints
Histology
Conventional Chordoma
Chondroid chordoma 2.4 2.7
Dedifferentiated chordoma 9.8 10.0
Surgical stage
Localized
Surgery
Tumor size
Contents lists available at ScienceDirect
Journal of Molecular Structure
Application of silver oxide nanoparticles for the treatment of cancer
a Department of Physics, GC University, Allama Iqbal Road, 38000, Faisalabad, Pakistan
b Department of Physics, Allama Iqbal Open University, H-8, Islamabad, Pakistan
c Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
d Institute of Biomedical and Genetic, Engineering (IBGE), 24 Mauve Area, G-9/1, Islamabad, Pakistan
e Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
Article history:
Received in revised form
Keywords:
Hepatocellular model (HepG2 cell line)
Silver oxide nanoparticles
Cytotoxicity
Statistical analysis 
Silver oxide nanoparticles are wonderful material and having great potential towards biomedical ap-plications. Silver oxide nanoparticle were synthesized via Chemical Aqueous method and characterized by applying manifold available techniques. X-ray diffraction (XRD) was used for analyzing structural property of nanoparticle crystals, the morphology of synthesized nanoparticles was studied by scanning electron microscope (SEM), elemental analysis of the composition was observed by energy dispersive X-ray spectra (EDXS) and the optical properties was analyzed by the UveVis spectrometer. Spectroscopic analysis confirmed the spherical morphology of the nanoparticles with the effect of calcined tempera-ture. Phototoxic and cytotoxic effects of grown particles were examined by conducting various relevant experimental techniques on hepatocellular (HepG2 Cell line) model. The obtained results were verified by applying polynomial fit which confirmed the goodness of fit. Silver oxide NPs has unique bio inter-action characteristics and physicochemical properties as anticancer agent. This research will be beneficial particularly for cancer therapeutics.
1. Introduction
Nanoparticles gain much popularity due to biological, thera-peutic and medical applications in the present era [1]. Among the nanomaterials, silver oxide nanoparticles are much more popular in the fields of textile, food industry, electronics, construction ma-terials, pharmaceutical products, antimicrobial agent, cosmetic and paints [2,3]. The cost effective and environmental friendly synthesis of silver oxide nanoparticles is the hot topic of current research studies. Silver oxide nanoparticles have strong antibacterial, anti-viral and antifungal effects [4]. Nanoparticles are being rapidly used as nano medicine, nano therapeutic, nano theranostic and cancer treatment [5].